Sir2B, A Sirtuin Family Protein, Involves in Cell Adhesion Molecule Expression During Early Dictyostelium Development Upon Starvation
Hideo Taniura,
Shuhei Soeda,
Ai Fujii,
Mio Morioka,
Minori Nakamura,
Yui Sano
Issue:
Volume 9, Issue 1, June 2021
Pages:
1-6
Received:
4 January 2021
Accepted:
18 January 2021
Published:
25 January 2021
Abstract: Sirtuin involves in cellular processes to adapt to starvation in the endocrine and metabolic systems. We examined Sir2B function during early Dictyostelium development upon starvation. GFP-tagged Sir2B was expressed and the immunoreactivity was detected a punctate pattern near cell surface. Sir2B mRNA was expressed in vegetative cells and peaked at 2 h after starvation. Ectopic expression of Sir2B enhanced aggregate formation with increased expressions of cell adhesion molecules such as cadA, csaA, TgrB1, and TgrC1 at 8h compared to those in control cells. Sir2B null-mutant cells (Sir2B KO cells) were generated and they formed aggregates smaller than those of wild-type cells after 24 h. Because cell-cell adhesion affects aggregation size, cell cohesion assay of Sir2B KO cells showed cell-cell adhesion impairment at 8 and 10 h during development, and the expressions of four cell adhesion molecules were reduced in Sir2B KO cells compared to wild-type cells until 8 h after starvation. When RNAi-mediated cadA or csaA knockdown cells (cadA KD or csaA KD cells) were generated, csaA KD cells formed smaller aggregates and affected the expressions of other cell adhesion molecules to reduce, but cadA KD cells did not show to affect aggregation size and cell adhesion molecules expressions apparently. These results suggest that Sir2B is involved in csaA expression and affects other cell adhesion molecules during early development upon starvation.
Abstract: Sirtuin involves in cellular processes to adapt to starvation in the endocrine and metabolic systems. We examined Sir2B function during early Dictyostelium development upon starvation. GFP-tagged Sir2B was expressed and the immunoreactivity was detected a punctate pattern near cell surface. Sir2B mRNA was expressed in vegetative cells and peaked at...
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Evaluation of Biochemical, Hematological and Antioxidant Properties in Mice Exposed to a Triherbal (Nigella sativa, Carica papaya and Boswellia sacra) Formular
Kehinde Sowunmi,
Adebayo Sofiyyah Modupeola,
Adesiyan Ayobami Lawal,
Kade Emmanuel Ayomikun,
Gurpreet Kaur
Issue:
Volume 9, Issue 1, June 2021
Pages:
7-15
Received:
26 January 2021
Accepted:
6 February 2021
Published:
29 April 2021
Abstract: Nigella sativa, Carica papaya and Boswellia sacra are medicinal plants in the commonly used in folkloric medicine due to the presence of its immense therapeutic properties. Fifty (50) female albino mice weighing between 15-22g were divided into five groups of 10 mice each. Animal in group 1 served as control group and were administered distilled water while animal in group 2 were given 2ml of cisplatin (orally). Animal in group 3-5 were given orally; 100 mg/kg (low dose), 200 mg/kg (medium dose) and 400 mg/kg (high dose) of triherbal preparation. The feeding regimens lasted for 28 days. After 28 days, mammary gland and blood samples were collected for haematological and antioxidant analysis. The triherbal formula decreased the GSH and MDA levels of mice treated with 100 mg/kg and 400 mg/kg doses compare to control. The measurement of total protein content, SOD and CAT increased in treated animals compared to control. However, RBC (Red Blood Cell) counts significantly decreased in the low, medium and high dose groups (0.95±0.08, 6.57±0.08 and 3.55±0.55 x 106 cells/mm3 respectively) compared to control (7.34±0.40) at P<0.05. Also, significant decreases (P<0.05) in the level of the total WBC (White Blood Cell) count, platelet count, PCV (Packed Cell Volume) and Hb (haemoglobin) concentration were observed. The decreases were dose dependent. The MCH (Mean Corpuscular Haemoglobin) and MCHC (Mean Corpuscular Haemoglobin Concentration) except MCV (Mean Corpuscular Volume) significantly decreased in treated group only. The triherbal formulation exhibited significant antioxidant activities showing increased levels of SOD, CAT and Protein content due to activation of the enzyme involve in detoxification of free radicals and decreased in the level of GSH and MDA due to accumulation of peroxides and H2O2. Also, decreased in haematological parameters due to the presence of phytochemicals such as phenol, resins, saponins, sterols, tannis and terpenes in the triherbal formula. Therefore, it has potential to induce haematotoxicity hence consumption of high concentrations should be discouraged.
Abstract: Nigella sativa, Carica papaya and Boswellia sacra are medicinal plants in the commonly used in folkloric medicine due to the presence of its immense therapeutic properties. Fifty (50) female albino mice weighing between 15-22g were divided into five groups of 10 mice each. Animal in group 1 served as control group and were administered distilled wa...
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Application of Conventional Techniques for the Diagnosis of Staphylococcus lugdunensis in Clinical Microbiology
Leonor Aties López,
Milagros de la Caridad Milá Pascual,
José Antúnez Coca
Issue:
Volume 9, Issue 1, June 2021
Pages:
16-19
Received:
17 March 2021
Accepted:
15 April 2021
Published:
29 April 2021
Abstract: Introduction: the participation of coagulase negative staphylococci in human diseases is an important problem, particularly in hospital settings where these species often act as opportunistic pathogens. Objective: to show the results obtained after applying conventional techniques for its detection. Results: the tests Cumpling factor, Detection of hemolysin δ-like, acidification of the substrates trehalose, mannose, mannitol and inositol were applied, as well as the ornithine decarboxylation biochemical test and the behavior of antimicrobial sensitivity was taken into account. all being feasible for the diagnosis of this microorganism due to the results obtained. Conclusions: Staphylococcus lugdunensis is an atypical SCoN compared to the rest of the SCoNs, both due to its special virulence characteristics and its striking sensitivity to multiple antimicrobials, including penicillin sensitivity in many cases. Its diagnosis in clinical microbiology laboratories is simple, given the suspicion of this species by the positive compliance factor test, a complete identification of it must be carried out through the decarboxylation of ornithine, the acidification of mannose, as well as antimicrobial sensitivity, are useful tests to differentiate this microorganism from the rest of its species.
Abstract: Introduction: the participation of coagulase negative staphylococci in human diseases is an important problem, particularly in hospital settings where these species often act as opportunistic pathogens. Objective: to show the results obtained after applying conventional techniques for its detection. Results: the tests Cumpling factor, Detection of ...
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