The Effect of Supporting Electrolyte on Ion Permeability Through Red Blood Cell Membrane
Benevolent Orighomisan Atolaiye,
Titilayo Oluwayemisi Bamidele,
Douglas Uwagbale Edward-Ekpu
Issue:
Volume 5, Issue 2, March 2017
Pages:
12-16
Received:
23 February 2017
Accepted:
14 March 2017
Published:
21 March 2017
Abstract: This study was aimed at providing information on how Na sulphate, K sulphate, Ca sulphate and Mg sulphate supporting electrolytes affect the permeation of hypotonic Na chloride, K chloride, Ca chloride and Mg chloride ions through Red Blood Cell (RBC) membrane while the RBCs haemolyse using a UV/Vis spectrophotometer. The effects of the supporting electrolytes were deduced from the changes in their respective rates of permeation through RBC membranes during haemolysis. Na2SO4 added to NaCl and MgSO4 to MgCl2 resulted in a decrease in permeation rate of the resultant solutions while the addition of K2SO4 to KCl and the addition of CaSO4 to CaCl2 resulted in an increase in permeation rate. In this research there was no regular pattern of permeation and since NaSO4 and MgSO4 decreased NaCl and MgCl2 permeability respectively, then the increase in the permeability of KCl and CaCl2 may not be as a result of the increase of K+ and Ca2+ concentration in K2SO4+KCl solution and CaCl2+CaSO4 solution respectively.
Abstract: This study was aimed at providing information on how Na sulphate, K sulphate, Ca sulphate and Mg sulphate supporting electrolytes affect the permeation of hypotonic Na chloride, K chloride, Ca chloride and Mg chloride ions through Red Blood Cell (RBC) membrane while the RBCs haemolyse using a UV/Vis spectrophotometer. The effects of the supporting ...
Show More
LC-MS Analysis of Phenolic Acids, Flavonoids and Betanin from Spineless Opuntia ficus-indica Fruits
Imen Belhadj Slimen,
Mahmoud Mabrouk,
Chaabane Hanène,
Taha Najar,
Manef Abderrabba
Issue:
Volume 5, Issue 2, March 2017
Pages:
17-28
Received:
16 February 2017
Accepted:
11 March 2017
Published:
1 April 2017
Abstract: Opuntia ficus-indica belongs to the Cactaceae family and is widespread in semi-arid and arid regions. Cactus pears are known for their health promoting properties which are due to a variety of bioactive molecules. This study aims to identify and quantify phenolic acids, flavonoids and betanin from spineless Opuntia ficus-indica fruits. Fresh mature samples were crushed, and extracted with 50% aqueous ethanol. The identification process was carried out using a Shimadzu high performance liquid chromagraph equipped with a quadrupole mass spectrum. Quantification was made using calibration curves of analytical standards. 9 phenolic acids, 1 flavan-3-ol, 2 flavanones, 3 flavonols, 6 flavones and 2 betacyanins were identified. Important levels of betanin, quinic acid, cirsiliol, acacetin, transcinnamic acid, rutin and naringin were calculated. Our results indicated that Opuntia ficus-indica fruits are an important dietary source of phenolic compounds and betalains with high antioxidant capacity.
Abstract: Opuntia ficus-indica belongs to the Cactaceae family and is widespread in semi-arid and arid regions. Cactus pears are known for their health promoting properties which are due to a variety of bioactive molecules. This study aims to identify and quantify phenolic acids, flavonoids and betanin from spineless Opuntia ficus-indica fruits. Fresh mature...
Show More
