Nuclear M-CSF Accelerates DNA Replication and Cell Proliferation in HeLa Human Cervical Cancer Cells
Jian Tu,
Ting Xiong,
Shujing Teng,
Zhigang Zhou,
Xiaoyong Lei,
Shengsong Tang
Issue:
Volume 5, Issue 1, January 2017
Pages:
1-7
Received:
31 January 2017
Accepted:
20 February 2017
Published:
4 March 2017
Abstract: Macrophage colony-stimulating factor (M-CSF), also named colony-stimulating factor-1 (CSF-1), plays an important role in the process of proliferation and differentiation of the monocyte/macrophage lineage cells. Commonly, it is not easy to measure the expression of cellular M-CSF. However, recent studies have shown that M-CSF can be expressed at a high level in the cytoplasm and nuclei of some kinds of malignant tumors, which related to the poor prognosis. To explore the role and mechanism of nuclear M-CSF, in the present study we constructed the pCMV/nuc/M-CSF vector and transfected it into human HeLa nuclei. The results from our previous study indicated, M-CSF was stably expressed in HeLa nuclei, which were used as a model to determine the nuclear effects of M-CSF. There was a higher percentage of replicating nuclei in the transfected pCMV/nuc/M-CSF HeLa cells both in phase G1 and S. According to the data from the cell doubling time, antisense oligonucleotides and the experiments of the transplanted tumor in nude mice, nuclear M-CSF could promote the cell proliferation of HeLa cells both in vivo and in vitro. In conclusion, nuclear M-CSF could accelerate DNA replication and cell proliferation of cervical carcinoma.
Abstract: Macrophage colony-stimulating factor (M-CSF), also named colony-stimulating factor-1 (CSF-1), plays an important role in the process of proliferation and differentiation of the monocyte/macrophage lineage cells. Commonly, it is not easy to measure the expression of cellular M-CSF. However, recent studies have shown that M-CSF can be expressed at a ...
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Prevalence of Trichomonasvaginalis Among Female Patients Attending the General Hospital Nasarawa, Nasarawa State
Yusuf Hussaini,
Justina A. Adegda,
Ademu Abdulkadir,
Abubakar Suleiman,
Salawu E. Murtala,
Yakubu Jibrin,
Aliyu Yakubu
Issue:
Volume 5, Issue 1, January 2017
Pages:
8-11
Received:
10 January 2017
Accepted:
10 February 2017
Published:
18 March 2017
Abstract: Trichomonasvaginalis, the etiologic agent of Trichomoniasis is transmitted by sexual intercourse, and is associated with the presence of other sexually transmitted diseases. In Nigeria, Trichomonasvaginalis infections are not reportable to health agencies and there is dearth of data on the actual prevalence. This cohort study determines the prevalence of Trichomonasvaginalis among female patients attending General Hospital Nasarawa, NasarawaState.Wet mount of all swab samples was made to check for the presence of motility. Out of 100 vaginal swabs samples analyzed, an overallprevalence of 12.0% was recorded. The highest prevalence of 16.67% was recorded for the 31-35 years old, while the lowest prevalence of 7.67% was recorded for16-20 years old. 13.2% prevalence was recorded in single women while 8.70% prevalence was recorded in married women. Those with three or more sex partners had 75% prevalence, 50% prevalencewas reported for those with two sex partners, while 3.6% was reported for those with one sex partner Trichomoniasis remained endemic in many developing countries where control may only be possible by regular screening and treatment. Sexual promiscuity and age were important predisposing factors of infection distribution in the studied population.
Abstract: Trichomonasvaginalis, the etiologic agent of Trichomoniasis is transmitted by sexual intercourse, and is associated with the presence of other sexually transmitted diseases. In Nigeria, Trichomonasvaginalis infections are not reportable to health agencies and there is dearth of data on the actual prevalence. This cohort study determines the prevale...
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