D-Riboce-L-Cystein Maintained Testicular Integrity in Rats Model (<i>Rattus Novergicus</i>) Exposed to X-Ray

Dare Babatunde Joseph; Olayemi Olamide Samuel; Falana Benedict Abiola; Duru Francis I. Ogueri; Osinubi Abraham A. A.

doi:doi:10.11648/j.cb.20170504.12

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D-Riboce-L-Cystein Maintained Testicular Integrity in Rats Model (Rattus Novergicus) Exposed to X-Ray

Dare Babatunde Joseph, Olayemi Olamide Samuel, Falana Benedict Abiola, Duru Francis I. Ogueri, Osinubi Abraham A. A.

Published in Cell Biology (Volume 5, Issue 4)

Received: 12 March 2017 Accepted: 27 May 2017 Published: 18 July 2017

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Abstract

Oxidative stress mechanisms are involved in xenobiotic-induced testicular dysfunctions which consequently lead to male infertility, however, antioxidants work like a defense system, disarming free radicals. Therefore, this study is aimed at investigating the maintenance of testicular integrity using D-Riboce-L-cysteine on X–ray induced testicular damage in adult wistar rats (Rattus Novergicus). A total of 20 male rats were randomly selected into Five (5) groups of Four (4) animal each. Control animals received only water while treated animals include: animals induced with X–ray only; around the pelvic and perineum region at about 95 kv, 12.5 milliampere-seconds (mA.s), 50 focal field distance (FFD), animals treated with D-Riboce-L-cysteine at 30 mg/kg body weight of D-Riboce-L-cysteine before exposed to 95 kv, 12.5 milliampere-seconds (mA.s), 50 focal field distance (FFD) of X-ray, animals treated with 95kv, 12.5 milliampere-seconds (mA.s), 50 focal field distance (FFD) of X-ray per animal before receiving 30 mg/kg body weight of D-Riboce-L-cysteine and animals treated with 30 mg/kg body weight of D-Riboce-L-cysteine only. After 21 days of treatments, the animals were sacrificed, and the testes were excised following abdominal incision, fixed in Bouin’s fluids for histological observations and right testis was homogenized in 5% sucrose solution for determination of enzymes of carbohydrates metabolism. Sperm was obtained from the caudal part of the epididymis for analysis of sperm characteristics. Reduced sperm count, abnormal morphology and significant (p < 0.05) higher non motile sperm characterized the animals expose to X-ray. However, sperm characteristics was maintained in control animals (p < 0.05) and animals treated with D-Riboce-L-cysteine only. Reduced activities in enzyme of carbohydrate metabolism (G-6-PDH) and significant increase in the level of lipid peroxidation shown by the activities of MDA in the X-ray treated groups compared to animals treated with D-Riboce-L-cysteine (p < 0.05) and the control animals. Abnormal widening of the interstitial space, loss of the basal laminal, degeneration in spermatogonia with vacuolation. Loss of germinal epithelium of the seminiferous tubules were also observed in animals expose to X-ray. Exposure to X-ray disrupts spermatogenesis by disruption and depletion of the spermatids and spermatogonia population, which caused increase in testicular tissue damage and consequently, altered the sperm characteristics. D-Riboce-L-cysteine clearly demonstrated maintenance of testicular integrity and enhance sperm characteristics; indication of fertility enhancing ability.

Published in	Cell Biology (Volume 5, Issue 4)
DOI	10.11648/j.cb.20170504.12
Page(s)	38-44
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

X-Ray, Testes, Free Radicals, Antioxidants and Wistar Rats

References

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Dare Babatunde Joseph, Olayemi Olamide Samuel, Falana Benedict Abiola, Duru Francis I. Ogueri, Osinubi Abraham A. A. (2017). D-Riboce-L-Cystein Maintained Testicular Integrity in Rats Model (Rattus Novergicus) Exposed to X-Ray. Cell Biology, 5(4), 38-44. https://doi.org/10.11648/j.cb.20170504.12

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ACS Style

Dare Babatunde Joseph; Olayemi Olamide Samuel; Falana Benedict Abiola; Duru Francis I. Ogueri; Osinubi Abraham A. A. D-Riboce-L-Cystein Maintained Testicular Integrity in Rats Model (Rattus Novergicus) Exposed to X-Ray. Cell Biol. 2017, 5(4), 38-44. doi: 10.11648/j.cb.20170504.12

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AMA Style

Dare Babatunde Joseph, Olayemi Olamide Samuel, Falana Benedict Abiola, Duru Francis I. Ogueri, Osinubi Abraham A. A. D-Riboce-L-Cystein Maintained Testicular Integrity in Rats Model (Rattus Novergicus) Exposed to X-Ray. Cell Biol. 2017;5(4):38-44. doi: 10.11648/j.cb.20170504.12

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@article{10.11648/j.cb.20170504.12,
  author = {Dare Babatunde Joseph and Olayemi Olamide Samuel and Falana Benedict Abiola and Duru Francis I. Ogueri and Osinubi Abraham A. A.},
  title = {D-Riboce-L-Cystein Maintained Testicular Integrity in Rats Model (Rattus Novergicus) Exposed to X-Ray},
  journal = {Cell Biology},
  volume = {5},
  number = {4},
  pages = {38-44},
  doi = {10.11648/j.cb.20170504.12},
  url = {https://doi.org/10.11648/j.cb.20170504.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cb.20170504.12},
  abstract = {Oxidative stress mechanisms are involved in xenobiotic-induced testicular dysfunctions which consequently lead to male infertility, however, antioxidants work like a defense system, disarming free radicals. Therefore, this study is aimed at investigating the maintenance of testicular integrity using D-Riboce-L-cysteine on X–ray induced testicular damage in adult wistar rats (Rattus Novergicus). A total of 20 male rats were randomly selected into Five (5) groups of Four (4) animal each. Control animals received only water while treated animals include: animals induced with X–ray only; around the pelvic and perineum region at about 95 kv, 12.5 milliampere-seconds (mA.s), 50 focal field distance (FFD), animals treated with D-Riboce-L-cysteine at 30 mg/kg body weight of D-Riboce-L-cysteine before exposed to 95 kv, 12.5 milliampere-seconds (mA.s), 50 focal field distance (FFD) of X-ray, animals treated with 95kv, 12.5 milliampere-seconds (mA.s), 50 focal field distance (FFD) of X-ray per animal before receiving 30 mg/kg body weight of D-Riboce-L-cysteine and animals treated with 30 mg/kg body weight of D-Riboce-L-cysteine only. After 21 days of treatments, the animals were sacrificed, and the testes were excised following abdominal incision, fixed in Bouin’s fluids for histological observations and right testis was homogenized in 5% sucrose solution for determination of enzymes of carbohydrates metabolism. Sperm was obtained from the caudal part of the epididymis for analysis of sperm characteristics. Reduced sperm count, abnormal morphology and significant (p < 0.05) higher non motile sperm characterized the animals expose to X-ray. However, sperm characteristics was maintained in control animals (p < 0.05) and animals treated with D-Riboce-L-cysteine only. Reduced activities in enzyme of carbohydrate metabolism (G-6-PDH) and significant increase in the level of lipid peroxidation shown by the activities of MDA in the X-ray treated groups compared to animals treated with D-Riboce-L-cysteine (p < 0.05) and the control animals. Abnormal widening of the interstitial space, loss of the basal laminal, degeneration in spermatogonia with vacuolation. Loss of germinal epithelium of the seminiferous tubules were also observed in animals expose to X-ray. Exposure to X-ray disrupts spermatogenesis by disruption and depletion of the spermatids and spermatogonia population, which caused increase in testicular tissue damage and consequently, altered the sperm characteristics. D-Riboce-L-cysteine clearly demonstrated maintenance of testicular integrity and enhance sperm characteristics; indication of fertility enhancing ability.},
 year = {2017}
}

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TY  - JOUR
T1  - D-Riboce-L-Cystein Maintained Testicular Integrity in Rats Model (Rattus Novergicus) Exposed to X-Ray
AU  - Dare Babatunde Joseph
AU  - Olayemi Olamide Samuel
AU  - Falana Benedict Abiola
AU  - Duru Francis I. Ogueri
AU  - Osinubi Abraham A. A.
Y1  - 2017/07/18
PY  - 2017
N1  - https://doi.org/10.11648/j.cb.20170504.12
DO  - 10.11648/j.cb.20170504.12
T2  - Cell Biology
JF  - Cell Biology
JO  - Cell Biology
SP  - 38
EP  - 44
PB  - Science Publishing Group
SN  - 2330-0183
UR  - https://doi.org/10.11648/j.cb.20170504.12
AB  - Oxidative stress mechanisms are involved in xenobiotic-induced testicular dysfunctions which consequently lead to male infertility, however, antioxidants work like a defense system, disarming free radicals. Therefore, this study is aimed at investigating the maintenance of testicular integrity using D-Riboce-L-cysteine on X–ray induced testicular damage in adult wistar rats (Rattus Novergicus). A total of 20 male rats were randomly selected into Five (5) groups of Four (4) animal each. Control animals received only water while treated animals include: animals induced with X–ray only; around the pelvic and perineum region at about 95 kv, 12.5 milliampere-seconds (mA.s), 50 focal field distance (FFD), animals treated with D-Riboce-L-cysteine at 30 mg/kg body weight of D-Riboce-L-cysteine before exposed to 95 kv, 12.5 milliampere-seconds (mA.s), 50 focal field distance (FFD) of X-ray, animals treated with 95kv, 12.5 milliampere-seconds (mA.s), 50 focal field distance (FFD) of X-ray per animal before receiving 30 mg/kg body weight of D-Riboce-L-cysteine and animals treated with 30 mg/kg body weight of D-Riboce-L-cysteine only. After 21 days of treatments, the animals were sacrificed, and the testes were excised following abdominal incision, fixed in Bouin’s fluids for histological observations and right testis was homogenized in 5% sucrose solution for determination of enzymes of carbohydrates metabolism. Sperm was obtained from the caudal part of the epididymis for analysis of sperm characteristics. Reduced sperm count, abnormal morphology and significant (p < 0.05) higher non motile sperm characterized the animals expose to X-ray. However, sperm characteristics was maintained in control animals (p < 0.05) and animals treated with D-Riboce-L-cysteine only. Reduced activities in enzyme of carbohydrate metabolism (G-6-PDH) and significant increase in the level of lipid peroxidation shown by the activities of MDA in the X-ray treated groups compared to animals treated with D-Riboce-L-cysteine (p < 0.05) and the control animals. Abnormal widening of the interstitial space, loss of the basal laminal, degeneration in spermatogonia with vacuolation. Loss of germinal epithelium of the seminiferous tubules were also observed in animals expose to X-ray. Exposure to X-ray disrupts spermatogenesis by disruption and depletion of the spermatids and spermatogonia population, which caused increase in testicular tissue damage and consequently, altered the sperm characteristics. D-Riboce-L-cysteine clearly demonstrated maintenance of testicular integrity and enhance sperm characteristics; indication of fertility enhancing ability.
VL  - 5
IS  - 4
ER  -

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Author Information

Dare Babatunde Joseph

Anatomy Department, Osun State University, Osogbo, Nigeria
Olayemi Olamide Samuel

Anatomy Department, Osun State University, Osogbo, Nigeria
Falana Benedict Abiola

Anatomy Department, Osun State University, Osogbo, Nigeria
Duru Francis I. Ogueri

Anatomy Department, University of Lagos, Lagos, Nigeria
Osinubi Abraham A. A.

Anatomy Department, University of Lagos, Lagos, Nigeria

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APA Style

Dare Babatunde Joseph, Olayemi Olamide Samuel, Falana Benedict Abiola, Duru Francis I. Ogueri, Osinubi Abraham A. A. (2017). D-Riboce-L-Cystein Maintained Testicular Integrity in Rats Model (Rattus Novergicus) Exposed to X-Ray. Cell Biology, 5(4), 38-44. https://doi.org/10.11648/j.cb.20170504.12

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ACS Style

Dare Babatunde Joseph; Olayemi Olamide Samuel; Falana Benedict Abiola; Duru Francis I. Ogueri; Osinubi Abraham A. A. D-Riboce-L-Cystein Maintained Testicular Integrity in Rats Model (Rattus Novergicus) Exposed to X-Ray. Cell Biol. 2017, 5(4), 38-44. doi: 10.11648/j.cb.20170504.12

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AMA Style

Dare Babatunde Joseph, Olayemi Olamide Samuel, Falana Benedict Abiola, Duru Francis I. Ogueri, Osinubi Abraham A. A. D-Riboce-L-Cystein Maintained Testicular Integrity in Rats Model (Rattus Novergicus) Exposed to X-Ray. Cell Biol. 2017;5(4):38-44. doi: 10.11648/j.cb.20170504.12

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@article{10.11648/j.cb.20170504.12,
  author = {Dare Babatunde Joseph and Olayemi Olamide Samuel and Falana Benedict Abiola and Duru Francis I. Ogueri and Osinubi Abraham A. A.},
  title = {D-Riboce-L-Cystein Maintained Testicular Integrity in Rats Model (Rattus Novergicus) Exposed to X-Ray},
  journal = {Cell Biology},
  volume = {5},
  number = {4},
  pages = {38-44},
  doi = {10.11648/j.cb.20170504.12},
  url = {https://doi.org/10.11648/j.cb.20170504.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cb.20170504.12},
  abstract = {Oxidative stress mechanisms are involved in xenobiotic-induced testicular dysfunctions which consequently lead to male infertility, however, antioxidants work like a defense system, disarming free radicals. Therefore, this study is aimed at investigating the maintenance of testicular integrity using D-Riboce-L-cysteine on X–ray induced testicular damage in adult wistar rats (Rattus Novergicus). A total of 20 male rats were randomly selected into Five (5) groups of Four (4) animal each. Control animals received only water while treated animals include: animals induced with X–ray only; around the pelvic and perineum region at about 95 kv, 12.5 milliampere-seconds (mA.s), 50 focal field distance (FFD), animals treated with D-Riboce-L-cysteine at 30 mg/kg body weight of D-Riboce-L-cysteine before exposed to 95 kv, 12.5 milliampere-seconds (mA.s), 50 focal field distance (FFD) of X-ray, animals treated with 95kv, 12.5 milliampere-seconds (mA.s), 50 focal field distance (FFD) of X-ray per animal before receiving 30 mg/kg body weight of D-Riboce-L-cysteine and animals treated with 30 mg/kg body weight of D-Riboce-L-cysteine only. After 21 days of treatments, the animals were sacrificed, and the testes were excised following abdominal incision, fixed in Bouin’s fluids for histological observations and right testis was homogenized in 5% sucrose solution for determination of enzymes of carbohydrates metabolism. Sperm was obtained from the caudal part of the epididymis for analysis of sperm characteristics. Reduced sperm count, abnormal morphology and significant (p < 0.05) higher non motile sperm characterized the animals expose to X-ray. However, sperm characteristics was maintained in control animals (p < 0.05) and animals treated with D-Riboce-L-cysteine only. Reduced activities in enzyme of carbohydrate metabolism (G-6-PDH) and significant increase in the level of lipid peroxidation shown by the activities of MDA in the X-ray treated groups compared to animals treated with D-Riboce-L-cysteine (p < 0.05) and the control animals. Abnormal widening of the interstitial space, loss of the basal laminal, degeneration in spermatogonia with vacuolation. Loss of germinal epithelium of the seminiferous tubules were also observed in animals expose to X-ray. Exposure to X-ray disrupts spermatogenesis by disruption and depletion of the spermatids and spermatogonia population, which caused increase in testicular tissue damage and consequently, altered the sperm characteristics. D-Riboce-L-cysteine clearly demonstrated maintenance of testicular integrity and enhance sperm characteristics; indication of fertility enhancing ability.},
 year = {2017}
}

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TY  - JOUR
T1  - D-Riboce-L-Cystein Maintained Testicular Integrity in Rats Model (Rattus Novergicus) Exposed to X-Ray
AU  - Dare Babatunde Joseph
AU  - Olayemi Olamide Samuel
AU  - Falana Benedict Abiola
AU  - Duru Francis I. Ogueri
AU  - Osinubi Abraham A. A.
Y1  - 2017/07/18
PY  - 2017
N1  - https://doi.org/10.11648/j.cb.20170504.12
DO  - 10.11648/j.cb.20170504.12
T2  - Cell Biology
JF  - Cell Biology
JO  - Cell Biology
SP  - 38
EP  - 44
PB  - Science Publishing Group
SN  - 2330-0183
UR  - https://doi.org/10.11648/j.cb.20170504.12
AB  - Oxidative stress mechanisms are involved in xenobiotic-induced testicular dysfunctions which consequently lead to male infertility, however, antioxidants work like a defense system, disarming free radicals. Therefore, this study is aimed at investigating the maintenance of testicular integrity using D-Riboce-L-cysteine on X–ray induced testicular damage in adult wistar rats (Rattus Novergicus). A total of 20 male rats were randomly selected into Five (5) groups of Four (4) animal each. Control animals received only water while treated animals include: animals induced with X–ray only; around the pelvic and perineum region at about 95 kv, 12.5 milliampere-seconds (mA.s), 50 focal field distance (FFD), animals treated with D-Riboce-L-cysteine at 30 mg/kg body weight of D-Riboce-L-cysteine before exposed to 95 kv, 12.5 milliampere-seconds (mA.s), 50 focal field distance (FFD) of X-ray, animals treated with 95kv, 12.5 milliampere-seconds (mA.s), 50 focal field distance (FFD) of X-ray per animal before receiving 30 mg/kg body weight of D-Riboce-L-cysteine and animals treated with 30 mg/kg body weight of D-Riboce-L-cysteine only. After 21 days of treatments, the animals were sacrificed, and the testes were excised following abdominal incision, fixed in Bouin’s fluids for histological observations and right testis was homogenized in 5% sucrose solution for determination of enzymes of carbohydrates metabolism. Sperm was obtained from the caudal part of the epididymis for analysis of sperm characteristics. Reduced sperm count, abnormal morphology and significant (p < 0.05) higher non motile sperm characterized the animals expose to X-ray. However, sperm characteristics was maintained in control animals (p < 0.05) and animals treated with D-Riboce-L-cysteine only. Reduced activities in enzyme of carbohydrate metabolism (G-6-PDH) and significant increase in the level of lipid peroxidation shown by the activities of MDA in the X-ray treated groups compared to animals treated with D-Riboce-L-cysteine (p < 0.05) and the control animals. Abnormal widening of the interstitial space, loss of the basal laminal, degeneration in spermatogonia with vacuolation. Loss of germinal epithelium of the seminiferous tubules were also observed in animals expose to X-ray. Exposure to X-ray disrupts spermatogenesis by disruption and depletion of the spermatids and spermatogonia population, which caused increase in testicular tissue damage and consequently, altered the sperm characteristics. D-Riboce-L-cysteine clearly demonstrated maintenance of testicular integrity and enhance sperm characteristics; indication of fertility enhancing ability.
VL  - 5
IS  - 4
ER  -

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