Korarima (Aframomum corrorima (Braun) P.C.M. Jansen) is a diploid, 2n=48, monocious, perennial and aromatic herb species under family Zingiberaceae. It is a native plant preferred as an aromatic in regular Ethiopian serving dishes and locally choice in traditional medicine. However, commercial exploitation of this crop is hampered due to poor rate of seed germination and slow multiplication rate of the rhizomes along with other agronomic predicaments. Thus, the present study was aimed at in vitro micro propagations of two Aframomum corrorima cultivars of Bonga1 and Benchmajji1. Based on the results, better response was observed in rhizome bud explants with the highest mean number of micro shoots per explants, 4.33±0.33 and 4.67±0.33 for cv. Bonga1 and Benchmajji1 respectively, whereas 3.33±0.33 and 3.00±0.00 for cv. Bonga1 and Benchmajji1 respectively on the MS basal medium added BA 2.0mg/l and KN 0.5 mg/l. For multiplication, the highest mean number of shoots per explants (4.67±0.33) was obtained from the MS medium supplemented with combination of 3.0mg/l BA and 0.5 mg/l KN for cv. Bonga1, and the highest mean number of shoots per explants (4.00±0.00) has been found from the MS medium enhanced with 2.0mg/l BA from shoot tip explants. For rooting induction, the highest mean number of roots per explants (15.00±1.15) was obtained in the MS supplemented with 1.0mg/l NAA for cv. Bonga1, and the highest mean number of roots per explants (17.33±0.88) was observed in the MS supplemented with 1.5mg/l IBA and 2.0mg/l for genotype cv. Benchmajji1, but the highest root length was obtained in ex vitro rooting with sand and vermi-compost at ratio of 2:1 with 1mg/l IBA three times per a week.
| Published in | Cell Biology (Volume 10, Issue 1) |
| DOI | 10.11648/j.cb.20221001.12 |
| Page(s) | 16-24 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
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Copyright © The Author(s), 2022. Published by Science Publishing Group |
In vitro Clonal Propagation, Shoot Multiplication, Rooting Induction and Ex-vitro
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APA Style
Berihu Mengs. (2022). In vitro Clonal Propagation of Korarima (Aframomum corrorima (Braun) P.C.M. Jansen) on Improved Varieties of Bonga1 and Benchmajji1. Cell Biology, 10(1), 16-24. https://doi.org/10.11648/j.cb.20221001.12
ACS Style
Berihu Mengs. In vitro Clonal Propagation of Korarima (Aframomum corrorima (Braun) P.C.M. Jansen) on Improved Varieties of Bonga1 and Benchmajji1. Cell Biol. 2022, 10(1), 16-24. doi: 10.11648/j.cb.20221001.12
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Download@article{10.11648/j.cb.20221001.12,
author = {Berihu Mengs},
title = {In vitro Clonal Propagation of Korarima (Aframomum corrorima (Braun) P.C.M. Jansen) on Improved Varieties of Bonga1 and Benchmajji1},
journal = {Cell Biology},
volume = {10},
number = {1},
pages = {16-24},
doi = {10.11648/j.cb.20221001.12},
url = {https://doi.org/10.11648/j.cb.20221001.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cb.20221001.12},
abstract = {Korarima (Aframomum corrorima (Braun) P.C.M. Jansen) is a diploid, 2n=48, monocious, perennial and aromatic herb species under family Zingiberaceae. It is a native plant preferred as an aromatic in regular Ethiopian serving dishes and locally choice in traditional medicine. However, commercial exploitation of this crop is hampered due to poor rate of seed germination and slow multiplication rate of the rhizomes along with other agronomic predicaments. Thus, the present study was aimed at in vitro micro propagations of two Aframomum corrorima cultivars of Bonga1 and Benchmajji1. Based on the results, better response was observed in rhizome bud explants with the highest mean number of micro shoots per explants, 4.33±0.33 and 4.67±0.33 for cv. Bonga1 and Benchmajji1 respectively, whereas 3.33±0.33 and 3.00±0.00 for cv. Bonga1 and Benchmajji1 respectively on the MS basal medium added BA 2.0mg/l and KN 0.5 mg/l. For multiplication, the highest mean number of shoots per explants (4.67±0.33) was obtained from the MS medium supplemented with combination of 3.0mg/l BA and 0.5 mg/l KN for cv. Bonga1, and the highest mean number of shoots per explants (4.00±0.00) has been found from the MS medium enhanced with 2.0mg/l BA from shoot tip explants. For rooting induction, the highest mean number of roots per explants (15.00±1.15) was obtained in the MS supplemented with 1.0mg/l NAA for cv. Bonga1, and the highest mean number of roots per explants (17.33±0.88) was observed in the MS supplemented with 1.5mg/l IBA and 2.0mg/l for genotype cv. Benchmajji1, but the highest root length was obtained in ex vitro rooting with sand and vermi-compost at ratio of 2:1 with 1mg/l IBA three times per a week.},
year = {2022}
}
TY - JOUR T1 - In vitro Clonal Propagation of Korarima (Aframomum corrorima (Braun) P.C.M. Jansen) on Improved Varieties of Bonga1 and Benchmajji1 AU - Berihu Mengs Y1 - 2022/04/28 PY - 2022 N1 - https://doi.org/10.11648/j.cb.20221001.12 DO - 10.11648/j.cb.20221001.12 T2 - Cell Biology JF - Cell Biology JO - Cell Biology SP - 16 EP - 24 PB - Science Publishing Group SN - 2330-0183 UR - https://doi.org/10.11648/j.cb.20221001.12 AB - Korarima (Aframomum corrorima (Braun) P.C.M. Jansen) is a diploid, 2n=48, monocious, perennial and aromatic herb species under family Zingiberaceae. It is a native plant preferred as an aromatic in regular Ethiopian serving dishes and locally choice in traditional medicine. However, commercial exploitation of this crop is hampered due to poor rate of seed germination and slow multiplication rate of the rhizomes along with other agronomic predicaments. Thus, the present study was aimed at in vitro micro propagations of two Aframomum corrorima cultivars of Bonga1 and Benchmajji1. Based on the results, better response was observed in rhizome bud explants with the highest mean number of micro shoots per explants, 4.33±0.33 and 4.67±0.33 for cv. Bonga1 and Benchmajji1 respectively, whereas 3.33±0.33 and 3.00±0.00 for cv. Bonga1 and Benchmajji1 respectively on the MS basal medium added BA 2.0mg/l and KN 0.5 mg/l. For multiplication, the highest mean number of shoots per explants (4.67±0.33) was obtained from the MS medium supplemented with combination of 3.0mg/l BA and 0.5 mg/l KN for cv. Bonga1, and the highest mean number of shoots per explants (4.00±0.00) has been found from the MS medium enhanced with 2.0mg/l BA from shoot tip explants. For rooting induction, the highest mean number of roots per explants (15.00±1.15) was obtained in the MS supplemented with 1.0mg/l NAA for cv. Bonga1, and the highest mean number of roots per explants (17.33±0.88) was observed in the MS supplemented with 1.5mg/l IBA and 2.0mg/l for genotype cv. Benchmajji1, but the highest root length was obtained in ex vitro rooting with sand and vermi-compost at ratio of 2:1 with 1mg/l IBA three times per a week. VL - 10 IS - 1 ER -
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